The NDX nanopore chips will lead to advancement of technology on many fronts including high throughput single molecule and single cell analysis.
Biological nanopores have evolved over millions of years to perform specialized functions. Pathogens such as bacteria and viruses routinely use nanopores to transport biomolecules into the host. Mimicking these processes ex situ allows for the creation of novel sensing and analytical technologies. In fact, miniaturization to the nanometer scale opens up the possibility to probe biology on the length scale where fundamental biological processes take place, such as epigenetic and genetic control of single cells. We re very much interested in biologically inspired nanopore systems fabricated by novel nanofabrication techniques for single molecule analysis to achieve ultra-fast high throughput DNA sequencing and non-time consuming low concentration detection of pathogenic single cells. The NDX nanopore chips include single and multiple nanopores on free-standing singular- and multi-layer 2D materials, such as graphene, h-BN, and graphene/h-BN. The NDX nanopore chips are continuously improving our understanding of biological macromolecules and are envisioned to be the next paradigm of genome sequencing technology. Single molecule sensing technologies are able to yield critically important information about molecular properties and functions for an analyte, even when it is present among a highly complex and heterogeneous molecular population. However, achieving acceptable resolution, signal-to-noise ratio, reliability and cost effective manufacturing are major challenges for all such platforms. The NDX Inc. proves the ability of our nanopore chips using 2D materials to detect small sequence specific probes, and will lead to significant improvement in DNA sequencing techniques for capturing comprehensive genetic data with high temporal resolution.